Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/1885
Title: Estudio retrospectivo de la aplicación de la amplificación génica cualitativa en muestras biológicas para el seguimiento de la toxoplasmosis en pacientes pediátricos receptores de trasplante de células progenitoras hematopoyéticas
Other Titles: Retrospective study of the implementation of the qualitative PCR technique in biological samples for monitoring toxoplasmosis in pediatric patients receiving hematopoietic stem cell transplantation
Authors: Nigro, Mónica G 
Figueroa, Carlos 
Ledesma, Bibiana A 
Keywords: Reacción en Cadena de la Polimerasa;Toxoplasma;Adolescente;Aloinjertos;Antiinfecciosos;Antiprotozoarios;Niño;Preescolar;Clindamicina;Contraindicaciones;ADN Protozoario;Reacciones Falso Negativas;Reacciones Falso Positivas;Femenino;Humanos;Huésped Inmunocomprometido;Inmunosupresores;Masculino;Infecciones Oportunistas;Complicaciones Posoperatorias;Valor Predictivo de las Pruebas;Premedicación;Pirimetamina;Estudios Retrospectivos;Donantes de Tejidos;Toxoplasmosis;Combinación Trimetoprim y Sulfametoxazol;Trasplante de Células Madre Hematopoyéticas
Issue Date: Jan-2014
Publisher: Asociación Argentina de Microbiología
Project: datasets
Journal: Revista Argentina de microbiologia 
Abstract: 
Toxoplasmosis is an opportunistic infection caused by the parasite Toxoplasma gondii. The infection is severe and difficult to diagnose in patients receiving allogeneic hematopoietic stem cell transplantation (HSCT). Twelve patients receiving HSCT were monitored post-transplant, by qualitative PCR at the Children's Hospital S.A.M.I.C. "Prof. Dr. Juan P. Garrahan". The monitoring of these patients was defined by a history of positive serology for toxoplasmosis in the donor or recipient and because their hematologic condition did not allow the use of trimethoprim-sulfamethoxazole for prophylaxis. During the patients' monitoring, two of them with positive PCR results showed signs of illness by T. gondii and were treated with pyrimethamine-clindamycin. In two other patients, toxoplasmosis was the cause of death and an autopsy finding, showing negative PCR results. Four patients without clinical manifestations received treatment for toxoplasmosis because of positive PCR detection. In four patients there were no signs of toxoplasmosis disease and negative PCR results during follow-up. The qualitative PCR technique proved useful for the detection of toxoplasmosis reactivation in HSCT recipients, but has limitations in monitoring and making clinical decisions due to the persistence of positive PCR over time and manifestations of toxicity caused by the treatment.
Description: 
Fil: Nigro, Mónica G. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.

Fil: Figueroa, Carlos. Hospital de Pediatría S.A.M.I.C. "Profesor Dr. Juan P. Garrahan". Servicio de Trasplante de Médula Ósea; Argentina.

Fil: Ledesma, Bibiana A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
URI: http://sgc.anlis.gob.ar/handle/123456789/1885
ISSN: 0325-7541
DOI: 10.1016/S0325-7541(14)70043-4
Rights: Open Access
Appears in Collections:Publicaciones INEI

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