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Title: | Epstein-Barr virus load in transplant patients: Early detection of post-transplant lymphoproliferative disorders | Other Titles: | Carga de virus Epstein-Barr en pacientes trasplantados: detección temprana de desórdenes linfoproliferativos postrasplante | Authors: | Fellner, María Dolores Durand, Karina A Solernou, Veronica Bosaleh, Andrea Balbarrey, Ziomara García de Dávila, María T Rodriguez, Marcelo Irazu, Lucía Alonio, Lidia V. Picconi, María Alejandra |
Keywords: | Carga viral;Linfoma;Reacción en Cadena en Tiempo Real de la Polimerasa;Herpesvirus Humano 4;Niño;Preescolar;Estudios Transversales;ADN Viral;Detección Precoz del Cáncer;Infecciones por Virus de Epstein-Barr;Estudios de Seguimiento;Humanos;Huésped Inmunocomprometido;Lactante;Leucocitos Mononucleares;Tejido Linfoide;Linfoma;Trastornos Linfoproliferativos;Complicaciones Posoperatorias;Estudios Retrospectivos;Viremia;Trasplante de Corazón;Trasplante de Riñón;Trasplante de Hígado | Issue Date: | Apr-2016 | Publisher: | Asociación Argentina de Microbiología | Project: | datasets | Journal: | Revista Argentina de microbiologia | Abstract: | High levels of circulating EBV load are used as a marker of post-transplant lymphoproliferative disorders (PTLD). There is no consensus regarding the threshold level indicative of an increase in peripheral EBV DNA. The aim of the study was to clinically validate a developed EBV quantification assay for early PTLD detection. Transversal study: paired peripheral blood mononuclear cells (PBMC), plasma and oropharyngeal lymphoid tissue (OLT) from children undergoing a solid organ transplant with (n=58) and without (n=47) PTLD. Retrospective follow-up: 71 paired PBMC and plasma from recipients with (n=6) and without (n=6) PTLD history. EBV load was determined by real-time PCR. The diagnostic ability to detect all PTLD (categories 1-4), advanced PTLD (categories 2-4) or neoplastic PTLD (categories 3 and 4) was estimated by analyzing the test performance at different cut-off values or with a load variation greater than 0.5log units. The higher diagnostic performance for identifying all, advanced or neoplastic PTLD, was achieved with cut-off values of 1.08; 1.60 and 2.47log EBVgEq/10(5) PBMC or 2.30; 2.60; 4.47loggEq/10(5) OLT cells, respectively. EBV DNA detection in plasma showed high specificity but low (all categories) or high (advanced/neoplastic categories) sensitivity for PTLD identification. Diagnostic performance was greater when: (1) a load variation in PBMC or plasma was identified; (2) combining the measure of EBV load in PBMC and plasma. The best diagnostic ability to identify early PTLD stages was achieved by monitoring EBV load in PBMC and plasma simultaneously; an algorithm was proposed. |
Description: | Fil: Fellner, María Dolores. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Durand, Karina A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Solernou, Veronica. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina. Fil: Bosaleh, Andrea. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina. Fil: Balbarrey, Ziomara. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina. Fil: García de Dávila, María T. "Prof. Dr. Juan. P. Garrahan" National Pediatrics Hospital. Pathology Service; Argentina. Fil: Rodríguez, Marcelo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Irazu, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Alonio, Lidia V. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Picconi, María A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/1864 | ISSN: | 0325-7541 | DOI: | 10.1016/j.ram.2016.02.006 | Rights: | Open Access |
Appears in Collections: | Publicaciones INEI |
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Revista Argentina de Microbiología_2016_48_2_p110-p118.pdf | Artículo en inglés | 948.45 kB | Adobe PDF | View/Open |
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