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Title: | Primer aislamiento ambiental de Cryptococcus neoformans var. gattii en Argentina | Other Titles: | 1st environmental isolation of Cryptococcus neoformans var. gattii in Argentina | Authors: | Davel, Graciela Odelsia Abrantes, Ruben Antonio Brudny, M Córdoba, Susana Rodero, Laura Canteros, Cristina Perrotta, Diego |
Keywords: | Cryptococcus;Cryptococcus neoformans;Argentina | Issue Date: | 2003 | Journal: | Revista Argentina de microbiologia | Abstract: | Two strains of C. neoformans var. gattii serotype B were isolated from a park in Buenos Aires city, Argentina. Samples were collected in spring, rubbing with swabs on the inner hollow of Eucalyptus spp. and other trees, which were not identified at the collection moment. Samples were swabbed directly onto cafeic acid with antibiotic agar, then incubated at 28 degrees C and observed daily for a week. Isolates were recovered from cafeic acid medium showing characteristic colonies of the species that allowed differentiation from other microorganisms of the sample. Identification was performed using carbohydrate fermentation, assimilation of nitrogen and carbon sources, and urease and phenoloxidase detection. Strain variety was determined with canavanine-glicine-bromotimol blue agar medium (CGB). Genotypic characterization using AP-PCR with (GACA)4 aleatory primer showed that both band profiles could be differentiated by only one band. These two strains were isolated from tree species different from Eucalyptus spp. These isolates from natural sources demonstrate that C. neoformans var. gattii efosts in Buenos Aires city environment and that it is not necessarily associated with Eucalyptus spp. The presence of C. neoformansvar. gattii in the environment, detected for the first time in Argentina, may explain its sporadic association with human infections in the local population. |
Description: | Fil: Davel, Graciela Odelsia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina. Fil: Abrantes, R. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina. Fil: Brudny, M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina. Fil: Rodero, L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina. Fil: Canteros, Cristina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/1853 | ISSN: | 0325-7541 | Rights: | Open Access Creative Commons Attribution 4.0 International License |
Appears in Collections: | Publicaciones INEI |
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Rev Arg Micro_2003_35_21_110-112.pdf | Artículo en español | 126.09 kB | Adobe PDF | View/Open |
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