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Título: | Virulence profile comparison between LEE-negative Shiga toxin-producing Escherichia coli (STEC) strains isolated from cattle and humans | Autor(es): | Galli, Lucía Miliwebsky, Elizabeth Irino, Kinue Leotta, Gerardo A Rivas, Marta |
Palavras-chave: | Virulencia;Toxina Shiga;Escherichia coli | Data do documento: | 14-Jul-2010 | Editora: | Elsevier | Jornal: | Veterinary microbiology | Resumo: | For comparison purposes, the prevalence of 8 virulence markers was investigated, by PCR, in 153 cattle and 47 human Locus for Enterocyte Effacement (LEE)-negative Shiga toxin-producing Escherichia coli (STEC) strains isolated in Argentina. Also, their correlation with severe disease was established. The virulence markers studied comprises 5 fimbrial and nonfimbrial adhesin-encoding genes (fimA, iha, efa1, lpfA(O113), and saa) and 3 toxin genes (cdt-V, subAB and astA) in addition to the Shiga toxins. The most prevalent virulence marker found was that encoded by the lpfA(O113) gene (199/200, 99%). Comparatively, the lpfA(O113), fimA, iha, saa, subAB, cdt-V and astA genes were detected in 100%, 92.8%, 85%, 52.9%, 36%, 11.8% and 9.8% of the cattle strains and in 97.9%, 95.7%, 89.4%, 40.4%, 32%, 17% and 10.6% of the human strains, respectively. All STEC strains were efa1 negative. The most prevalent profile observed among cattle and human STEC strains was lpfA(O113)iha fimA. These results show that bovine LEE-negative STEC strains possessed genes encoding virulence factors present in human LEE-negative STEC strains that are associated with disease. Despite a great diversity of virulence profiles observed, further studies comparing wild type strains and their allelic mutants are needed to evaluate the role of each factor in the pathogenesis of LEE-negative STEC strains during human infections. |
Descrição: | Fil: Galli, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina. Fil: Miliwebsky, Elizabeth. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina. Fil: Irino, Kinue. Departamento de Microbiologia, Setor de Enterobactérias, Instituto Adolfo Lutz; Brasil. Fil: Leotta, Gerardo A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina. Fil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/1829 | ISSN: | 0378-1135 | DOI: | 10.1016/j.vetmic.2009.11.028 | Direitos: | Closed Access |
Aparece nas Coleções: | Publicaciones INEI |
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