Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/1816
Title: In vitro infection by different strains of Paracoccidioides brasiliensis
Authors: Canteros, Cristina Elena 
Soria, Marcelo 
Rivas, María Cristina 
Pérez, Celeste Luján 
Tous, Mónica 
Lee, William 
Rodero, Laura 
Davel, Graciela Odelsia 
Keywords: Animales;Chlorocebus aethiops;Medios de Cultivo;Proteínas Fúngicas;Glicoproteínas;Micología;Oligosacáridos;Paracoccidioides;Especificidad de la Especie;Células Vero;Virulencia;Antígenos Fúngicos
Issue Date: 2000
Journal: Revista Argentina de microbiologia 
Abstract: 
We analyzed the in vitro infection process by P. brasiliensis and the effect of extracellular factor(s) produced on monolayers of mammalian Vero cell lines. The yeast phase of four strains was studied: B339 (avirulent or slightly virulent), U, (intermediate virulence), 93745 and 63265 (both highly virulent). Strains of intermediate and high virulence had higher adherence at first contact (about 16%). Strain B339 had a slower adherence at first contact (8%) than the others during the same period. The production of extracellular proteases, soluble extracellular factor(s) and extracellular antigen gP43 showed no correlation with the in vitro physiopathogenicity of the analyzed strains. We demonstrate that the Vero model presented in this paper is a suitable system to study infection and virulence in vitro. We are currently assessing its usefulness as a tool for the analysis of the interaction between pathogen, host and antifungal agents.
Description: 
Fil: Canteros, C. E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.

Fil: Davel, Graciela Odelsia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
URI: http://sgc.anlis.gob.ar/handle/123456789/1816
ISSN: 0325-7541
Rights: Open Access
Attribution-NonCommercial-NoDerivs 3.0 Unported
Appears in Collections:Publicaciones INEI

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