Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/1706
Title: Do commercial serologic tests for Trypanosoma cruzi infection detect Mexican strains in women and newborns?
Authors: Gamboa-León, Miriam 
Gonzalez-Ramirez, Claudia 
Padilla-Raygoza, Nicolás 
Sosa-Estani, Sergio 
Caamal-Kantun, Alejandra 
Buekens, Pierre 
Dumonteil, Eric 
Keywords: Adulto;Anticuerpos Antiprotozoarios;Antígenos de Protozoos;Western Blotting;Enfermedad de Chagas;Estudios Transversales;Ensayo de Inmunoadsorción Enzimática;Sangre Fetal;Humanos;Recién Nacido;Transmisión Vertical de Enfermedad Infecciosa;México;Embarazo;Complicaciones Parasitarias del Embarazo;Sensibilidad y Especificidad;Estudios Seroepidemiológicos;Trypanosoma cruzi;Adulto Joven
Issue Date: Apr-2011
Publisher: American Society of Parasitologists
Project: datasets
Journal: The Journal of parasitology 
Abstract: 
We sought to determine the serological test that could be used for Trypanosoma cruzi seroprevalence studies in Mexico, where lineage I predominates. In a previous study among pregnant women and their newborns in the states of Yucatan and Guanajuato, we reported a 0.8-0.9% of prevalence for T. cruzi -specific antibodies by Stat-Pak and Wiener ELISA. We have expanded this study here by performing an additional non-commercial ELISA and confirming the seropositives with Western blot, using whole antigens of a local parasite strain. We found a seroprevalence of 0.6% (3/500) in Merida and 0.4% in Guanajuato (2/488). The 5 seropositive umbilical cord samples reacted to both non-commercial ELISA and Western blot tests, and only 1 of the maternal samples was not reactive to non-commercial ELISA. A follow-up of the newborns at 10 mo was performed in Yucatan to determine the presence of T. cruzi antibodies in children as evidence of congenital infection. None of the children was seropositive. One newborn from an infected mother died at 2 wk of age of cardiac arrest, but T. cruzi infection was not confirmed. The T. cruzi seroprevalence data obtained with both commercial tests (Stat-Pak and ELISA Wiener) are similar to those from non-commercial tests using a local Mexican strain of T. cruzi.
Description: 
Fil: Gamboa-León, Rubi. Tulane University. School of Public Health and Tropical Medicine; Estados Unidos.

Fil: González-Ramírez, Claudia. Universidad de Guanajuato. División Ciencias de la Salud e Ingenierías. Departamento de Enfermería y Obstetricia; México.

Fil: Padilla-Raygoza, Nicolás. Universidad de Guanajuato. División Ciencias de la Salud e Ingenierías. Departamento de Enfermería y Obstetricia; México.

Fil: Sosa-Estani, Sergio. ANLIS Dr.C.G.Malbrán. Centro Nacional de Diagnóstico e Investigación de Endemo-Epidemias; Argentina.

Fil: Caamal-Kantun, Alejandra. Tulane University. School of Public Health and Tropical Medicine; Estados Unidos.

Fil: Buekens, Pierre. Tulane University. School of Public Health and Tropical Medicine; Estados Unidos.

Fil: Dumonteil, Eric. Tulane University. School of Public Health and Tropical Medicine; Estados Unidos.
URI: http://sgc.anlis.gob.ar/handle/123456789/1706
DOI: 10.1645/GE-2545.1
Rights: Open Access
Appears in Collections:Publicaciones CeNDIE

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