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Title: | Desarrollo y evaluación de una base de datos in house para la identificación rápida de Burkholderia contaminans por EM MALDI-TOF | Authors: | Cipolla, Lucía Rocca, María Florencia Armitano, Rita Martínez, Claudia Almuzara, Marisa Faccone, Diego Vay, Carlos Prieto, Mónica A. |
Keywords: | Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción;Identificación molecular | Issue Date: | 2019 | Journal: | Revista Argentina de microbiologia | Abstract: | MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry (MS) proved to be a robust tool for the identification of numerous taxonomic groups. However, it has limitations. A key advantage of this technique is the flexibility for the incorporation of protein profiles of microorganisms not included in the commercial database. Due to the prevalence of Burkholderia contaminans in fibrocystic patients in Argentina and the fact that rapid and reliable microbiological diagnosis is crucial in them, MALDI-TOF MS emerges as a strategic tool. The aim of this work was to develop an additional database with peptide spectra of reference isolates of B. contaminans. This database demonstrated to be successful for the identification of 97% of the isolates analyzed. Therefore, MALDI-TOF MS with the extended database was a useful tool for the identification and differentiation of other related species to B. contaminans. |
Description: | Fil: Cipolla, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Rocca, María Florencia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Armitano, Rita. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Martínez, Claudia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Almuzara, Marisa. Hospital de Clínicas José de San Martín. Facultad de Farmacia y Bioquímica. Universidad de Buenos Aires; Argentina Fil: Faccone, Diego. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Vay, Carlos. Hospital de Clínicas José de San Martín. Facultad de Farmacia y Bioquímica. Universidad de Buenos Aires; Argentina Fil: Prieto, Mónica. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/1532 | ISSN: | 0325-7541 | DOI: | 10.1016/j.ram.2018.09.001 |
Appears in Collections: | Publicaciones INEI |
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1-s2.0-S0325754118301111-main.pdf | 505.32 kB | Adobe PDF | View/Open |
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