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Title: Desarrollo y evaluación de una base de datos in house para la identificación rápida de Burkholderia contaminans por EM MALDI-TOF
Authors: Cipolla, Lucía 
Rocca, María Florencia 
Armitano, Rita 
Martínez, Claudia 
Almuzara, Marisa 
Faccone, Diego 
Vay, Carlos 
Prieto, Mónica A. 
Keywords: Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción;Identificación molecular
Issue Date: 2019
Journal: Revista Argentina de microbiologia 
Abstract: MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry (MS) proved to be a robust tool for the identification of numerous taxonomic groups. However, it has limitations. A key advantage of this technique is the flexibility for the incorporation of protein profiles of microorganisms not included in the commercial database. Due to the prevalence of Burkholderia contaminans in fibrocystic patients in Argentina and the fact that rapid and reliable microbiological diagnosis is crucial in them, MALDI-TOF MS emerges as a strategic tool. The aim of this work was to develop an additional database with peptide spectra of reference isolates of B. contaminans. This database demonstrated to be successful for the identification of 97% of the isolates analyzed. Therefore, MALDI-TOF MS with the extended database was a useful tool for the identification and differentiation of other related species to B. contaminans.
ISSN: 0325-7541
DOI: 10.1016/j.ram.2018.09.001
Appears in Collections:Publicaciones INEI

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