Please use this identifier to cite or link to this item:
http://sgc.anlis.gob.ar/handle/123456789/1503| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Kamenetzky, Laura | es |
| dc.contributor.author | Canova, S G | es |
| dc.contributor.author | Guarnera, Eduardo | es |
| dc.contributor.author | Rosenzvit, Mara C. | es |
| dc.date.accessioned | 2019-12-12T20:36:37Z | - |
| dc.date.available | 2019-12-12T20:36:37Z | - |
| dc.date.issued | 2000-06 | - |
| dc.identifier.issn | 0014-4894 | - |
| dc.identifier.uri | http://sgc.anlis.gob.ar/handle/123456789/1503 | - |
| dc.description.abstract | A method for the isolation of Echinococcus granulosus DNA from germinal layers of hydatid cysts is described. The method includes a hexadecyltrimethylammonium bromide/chloroform extraction and an adsorption to diatomaceous earth suspension. DNA suitable for polymerase chain reaction was obtained and used for parasite strain determination by mitochondrial cytochrome c oxidase I gene sequencing. Fertile and nonfertile cyst isolates from sheep, cattle, pigs, and humans were characterized. Hitherto, no direct parasite strain characterization has been made on nonfertile hydatid cysts, whereas here we report that nonfertile hydatid cysts were produced by sheep strain (G1 genotype) in sheep, cattle, and humans and by pig strain (G7 genotype) in pigs. | es |
| dc.language.iso | en | es |
| dc.relation.ispartof | Experimental parasitology | es |
| dc.title | Echinococcus granulosus: DNA extraction from germinal layers allows strain determination in fertile and nonfertile hydatid cysts | es |
| dc.type | Artículo | es |
| dc.identifier.doi | 10.1006/expr.2000.4518 | - |
| item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
| item.cerifentitytype | Publications | - |
| item.grantfulltext | none | - |
| item.openairetype | Artículo | - |
| item.fulltext | No Fulltext | - |
| item.languageiso639-1 | en | - |
| crisitem.author.dept | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
| crisitem.author.dept | Instituto Nacional de Enfermedades Infecciosas (INEI) | - |
| crisitem.author.dept | Departamento de Parasitología | - |
| crisitem.author.dept | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
| crisitem.author.dept | Instituto Nacional de Parasitología (INP) | - |
| crisitem.author.parentorg | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
| crisitem.author.parentorg | Instituto Nacional de Enfermedades Infecciosas (INEI) | - |
| crisitem.author.parentorg | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
| Appears in Collections: | Publicaciones INP | |
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