Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/1457
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dc.contributor.authorFerrero, Maximiliano Ren_US
dc.contributor.authorSoprano, Luciana Len_US
dc.contributor.authorAcosta, Diana Men_US
dc.contributor.authorGarcía, Gabriela Aen_US
dc.contributor.authorEsteva, Monica Ien_US
dc.contributor.authorCouto, Alicia Sen_US
dc.contributor.authorDuschak, Vilma Gen_US
dc.date.accessioned2019-12-05T19:49:16Z-
dc.date.available2019-12-05T19:49:16Z-
dc.date.issued2014-09-
dc.identifier.urihttp://sgc.anlis.gob.ar/handle/123456789/1457-
dc.description.abstractSulfation, a post-translational modification which plays a key role in various biological processes, is inhibited by competition with chlorate. In Trypanosoma cruzi, the agent of Chagas' disease, sulfated structures have been described as part of glycolipids and we have reported sulfated high-mannose type oligosaccharides in the C-T domain of the cruzipain (Cz) glycoprotein. However, sulfation pathways have not been described yet in this parasite. Herein, we studied the effect of chlorate treatment on T. cruzi with the aim to gain some knowledge about sulfation metabolism and the role of sulfated molecules in this parasite. In chlorate-treated epimastigotes, immunoblotting with anti-sulfates enriched Cz IgGs (AS-enriched IgGs) showed Cz undersulfation. Accordingly, a Cz mobility shift toward higher isoelectric points was observed in 2D-PAGE probed with anti-Cz antibodies. Ultrastructural membrane abnormalities and a significant decrease of dark lipid reservosomes were shown by electron microscopy and a significant decrease in sulfatide levels was confirmed by TLC/UV-MALDI-TOF-MS analysis. Altogether, these results suggest T. cruzi sulfation occurs via PAPS. Sulfated epitopes in trypomastigote and amastigote forms were evidenced using AS-enriched IgGs by immunoblotting. Their presence on trypomastigotes surface was demonstrated by flow cytometry and IF with Cz/dCz specific antibodies. Interestingly, the percentage of infected cardiac HL-1 cells decreased 40% when using chlorate-treated trypomastigotes, suggesting sulfates are involved in the invasion process. The same effect was observed when cells were pre-incubated with dCz, dC-T or an anti-high mannose receptor (HMR) antibody, suggesting Cz sulfates and HMR are also involved in the infection process by T. cruzi.en_US
dc.language.isoenen_US
dc.relation.ispartofActa tropicaen_US
dc.subjectTrypanosoma cruzien_US
dc.subjectCruzipainen_US
dc.subjectProceso de invasiónen_US
dc.subjectSomatomedinasen_US
dc.subjectSulfoglicoesfingolípidosen_US
dc.titleEffects of chlorate on the sulfation process of Trypanosoma cruzi glycoconjugates. Implication of parasite sulfates in cellular invasionen_US
dc.typeArtículoen_US
dc.identifier.doi10.1016/j.actatropica.2014.05.014-
item.openairetypeArtículo-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
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