Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/1422
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dc.contributor.authorQuebrada Palacio, Luz Pes
dc.contributor.authorGonzalez, Mariela N.es
dc.contributor.authorHernandez-Vasquez, Yolandaes
dc.contributor.authorPerrone, Alina E.es
dc.contributor.authorParodi-Talice, Adrianaes
dc.contributor.authorBua, Jacquelinees
dc.contributor.authorPostan, Miriames
dc.date.accessioned2019-11-29T20:37:43Z-
dc.date.available2019-11-29T20:37:43Z-
dc.date.issued2018-09-05-
dc.identifier.issn1932-6203-
dc.identifier.urihttp://sgc.anlis.gob.ar/handle/123456789/1422-
dc.descriptionFil: Quebrada Palacio, Luz P. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.es
dc.descriptionFil: Gonzalez, Mariela N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología. Departamento de Investigación y Docencia; Argentina.es
dc.descriptionFil: Hernandez-Vasquez, Yolanda. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología. Departamento de Investigación y Docencia; Argentina.es
dc.descriptionFil: Perrone, Alina E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología. Departamento de Investigación y Docencia; Argentina.es
dc.descriptionFil: Parodi-Talice, Adriana. Universidad de la República. Facultad de Ciencias. Sección Genética. Instituto Pasteur de Montevideo. Unidad de Biología Molecular Montevideo; Uruguay.es
dc.descriptionFil: Bua, Jacqueline. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.es
dc.descriptionFil: Postan, Miriam. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.es
dc.description.abstractTrypanosoma cruzi is a genetically heterogeneous group of organisms that cause Chagas disease. It has been long suspected that the clinical outcome of the disease and response to therapeutic agents are, at least in part, related to the genetic characteristics of the parasite. Herein, we sought to validate the significance of the genotype of T. cruzi isolates recovered from patients with different clinical forms of Chagas disease living in Argentina on their biological behaviour and susceptibility to drugs. Genotype identification of the newly established isolates confirmed the reported predominance of TcV, with a minor frequency of TcI. Epimastigote sensitivity assays demonstrated marked dissimilar responses to benznidazole, nifurtimox, pentamidine and dihydroartemisinin in vitro. Two TcV isolates exhibiting divergent response to benznidazole in epimastigote assays were further tested for the expression of anti-oxidant proteins. Benznidazole-resistant BOL-FC10A epimastigotes had decreased expression of Old Yellow Enzyme and cytosolic superoxide dismutase, and overexpression of mitochondrial superoxide dismutase and tryparedoxin- 1, compared to benznidazole-susceptible AR-SE23C parasites. Drug sensitivity assays on intracellular amastigotes and trypomastigotes reproduced the higher susceptibility of AR-SE23C over BOL-FC10A parasites to benznidazole observed in epimastigotes assays. However, the susceptibility/resistance profile of amastigotes and trypomastigotes to nifurtimox, pentamidine and dihydroartemisinin varied markedly with respect to that of epimastigotes. C3H/He mice infected with AR-SE23C trypomastigotes had higher levels of parasitemia and mortality rate during the acute phase of infection compared to mice infected with BOL-FC10A trypomastigotes. Treatment of infected mice with benznidazole or nifurtimox was efficient to reduce patent parasitemia induced by either isolate. Nevertheless, qPCR performed at 70 dpi revealed parasite DNA in the blood of mice infected with AR-SE23C but not in BOL-FC10A infected mice. These results demonstrate high level of intra-type diversity which may represent an important obstacle for the testing of chemotherapeutic agents.es
dc.formatpdf-
dc.language.isoenes
dc.relation.ispartofPloS onees
dc.rightsOpen Access-
dc.rightsCreative Commons Attribution 4.0 International License-
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/-
dc.sourcePLoS One 2018;13(9):e0203462-
dc.subjectVariación Biológica Poblacionales
dc.subjectPruebas de Sensibilidad Microbianaes
dc.subjectTrypanosoma cruzies
dc.titlePhenotypic diversity and drug susceptibility of Trypanosoma cruzi TcV clinical isolateses
dc.typeArtículoes
dc.identifier.doi10.1371/journal.pone.0203462-
item.openairetypeArtículo-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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