Please use this identifier to cite or link to this item: http://sgc.anlis.gov.ar/handle/123456789/1373
Title: Simplified MLST scheme for direct typing of Leptospira human clinical samples
Authors: Varni, Vanina 
Chiani, Yosena 
Nagel, Ariel 
Ruybal, Paula 
Vanasco, Bibiana N. 
Caimi, Karina 
Keywords: MLST;Leptospira;Serogrupo;muestras clínicas
Issue Date: 2018
Journal: Pathogens and global health 
Abstract: 
Leptospirosis is a globally distributed zoonosis. Epidemiological data are scarce and present major challenge because of the varied clinical presentations. Multilocus Sequence Typing has already proven to be a robust molecular typing method providing accurate results for strain characterization. We have adapted our MLST scheme by reducing the set of loci to facilitate Leptospira typing directly from human clinical samples. The application of this 3-locus scheme provides Leptospira species and allelic profiles of the samples retaining the power of discrimination of the whole scheme. Moreover, an approach to the serogroups was also achieved. Our results contribute to the epidemiological study of Leptospirosis, since the direct typing on clinical specimens could detect and update allelic variants and serogroups present in a region. The simplified scheme allowed at the same time to take advantage of limited genetic material available in clinical samples that may increase the sources of information for epidemiological monitoring.
URI: http://sgc.anlis.gob.ar/handle/123456789/1373
DOI: 10.1080/20477724.2018.1480137
Appears in Collections:Publicaciones INER

Files in This Item:
File Description SizeFormat
ypgh-112-1480137.pdfArtículo en inglés620.79 kBAdobe PDFThumbnail
View/Open
Show full item record

Page view(s)

4
checked on Jul 4, 2020

Download(s)

2
checked on Jul 4, 2020

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.