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Title: PCR-Based Method for Shigella flexneri Serotyping: International Multicenter Validation
Authors: Brengi, Silvina P. 
Sun, Qiangzheng 
Bolaños, Hilda 
Duarte, Francisco 
Jenkins, Claire 
Pichel, Mariana 
Shahnaij, Mohammad 
Sowers, Evangeline G 
Strockbine, Nancy 
Talukder, Kaisar A 
Derado, Gordana 
Viñas, María R. 
Kam, Kai Man 
Xu, Jianguo 
Keywords: Reacción en Cadena de la Polimerasa;Shigella flexneri;Serotipos moleculares;Serogrupo
Issue Date: Apr-2019
Journal: Journal of clinical microbiology 
Abstract: Shigella spp. are a leading cause of human diarrheal disease worldwide, with Shigella flexneri being the most frequently isolated species in developing countries. This serogroup is presently classified into 19 serotypes worldwide. We report here a multicenter validation of a multiplex-PCR-based strategy previously developed by Q. Sun, R. Lan, Y. Wang, A. Zhao, et al. (J Clin Microbiol 49:3766-3770, 2011) for molecular serotyping of S. flexneri This study was performed by seven international laboratories, with a panel of 71 strains (researchers were blind to their identity) as well as 279 strains collected from each laboratory's own local culture collections. This collaborative work found a high extent of agreement among laboratories, calculated through interrater reliability (IRR) measures for the PCR test that proved its robustness. Agreement with the traditional method (serology) was also observed in all laboratories for 14 serotypes studied, while specific genetic events could be responsible for the discrepancies among methodologies in the other 5 serotypes, as determined by PCR product sequencing in most of the cases. This work provided an empirical framework that allowed the use of this molecular method to serotype S. flexneri and showed several advantages over the traditional method of serological typing. These advantages included overcoming the problem of availability of suitable antisera in testing laboratories as well as facilitating the analysis of multiple samples at the same time. The method is also less time-consuming for completion and easier to implement in routine laboratories. We recommend that this PCR be adopted, as it is a reliable diagnostic and characterization methodology that can be used globally for laboratory-based shigella surveillance.
DOI: 10.1128/JCM.01592-18
Appears in Collections:Publicaciones INEI

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