Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/1315
Title: Dimorphism and Dissemination of Histoplasma capsulatum in the Upper Respiratory Tract after Intranasal Infection of Bats and Mice with Mycelial Propagules
Authors: Suárez-Álvarez, Roberto O 
Sahaza, Jorge H 
Berzunza-Cruz, Miriam 
Becker, Ingeborg 
Curiel-Quesada, Everardo 
Pérez-Torres, Armando 
Reyes-Montes, María del Rocío 
Taylor, Maria Lucia 
Issue Date: Sep-2019
Journal: The American journal of tropical medicine and hygiene 
Abstract: This article describes, for the first time, the role of the nasal mucosa (NM) as the initial site for the Histoplasma capsulatum mycelial-to-yeast transition. The results highlight that yeasts may arrive to the cervical lymph nodes (CLN) via phagocytes. Bats and mice were intranasally infected with H. capsulatum mycelial propagules and they were killed 10, 20, and 40 minutes and 1, 2, and 3 hours after infection. The NM and the CLN were monitored for fungal presence. Yeasts compatible with H. capsulatum were detected within the NM and the CLN dendritic cells (DCs) 2-3 hours postinfection, using immunohistochemistry. Histoplasma capsulatum was re-isolated by culturing at 28°C from the CLN of both mammalian hosts 2-3 hours postinfection. Reverse transcription-polymerase chain reaction assays were designed to identify fungal dimorphism, using mycelial-specific (MS8) and yeast-specific (YPS3) gene expression. This strategy supported fast fungal dimorphism in vivo, which began in the NM 1 hour postinfection (a time point when MS8 and YPS3 genes were expressed) and it was completed at 3 hours (a time point when only the YPS3 transcripts were detected) in both bats and mice. The presence of intracellular yeasts in the nasal-associated lymphoid tissue (NALT), in the NM nonassociated with the NALT, and within the interdigitating DCs of the CLN suggests early fungal dissemination via the lymph vessels.
URI: http://sgc.anlis.gob.ar/handle/123456789/1315
DOI: 10.4269/ajtmh.18-0788
Appears in Collections:Publicaciones INEI

Show full item record

Page view(s)

5
checked on Dec 13, 2019

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.