Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/365
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dc.contributor.authorLópez, Marcelaes
dc.contributor.authorAlvarez, Claudiaes
dc.contributor.authorImaz, María Susanaes
dc.date.accessioned2012-11-16T14:23:04Z-
dc.date.available2012-11-16T14:23:04Z-
dc.date.issued2011-
dc.identifier.issn0120-4157-
dc.identifier.urihttp://www.revistabiomedica.org/index.php/biomedica/article/view/300/573-
dc.identifier.urihttp://sgc.anlis.gob.ar/handle/123456789/365-
dc.descriptionFil: López, Marcela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.es
dc.descriptionFil: Alvarez, Claudia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.es
dc.descriptionFil: Imaz, María Susana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Argentina.es
dc.description.abstractIntroduction.The performance of a drug susceptibility test may change when moving from the research stage to implementation on a population level in actual public health practice. Objective.The performance of a rapid drug susceptibility test was described for detecting multidrug-resistant Mycobacterium tuberculosis when implemented in the routine workflow of a low-resource reference laboratory. Materials and methods.A prospective study was done comparing the performance of the nitrate reductase assay with the conventional proportion method for rifampicin and isoniazid on 364 isolates were obtained from multidrug-resistant tuberculosis risk patients referred from diffrent Colombian laboratories. Results.When compared with the proportion method, the nitrate reductase assay sensitivity was 86.8% and 84.9% for rifampicin and isoniazid, respectively, whereas nitrate reductase assay specificity was 100% for isoniazid and rifampicin. Nitrate reductase assay sensitivity was significantly higher when the age of isolate was less than 70 days. A sensitivity of 94.4% dropped to 78.1% for rifampicin resistance for fresh and old isolates, respectively (Fisher exact test, p=0.05). For isoniazid resistance using fresh and old isolates, 94.7% vs.74.3% sensitivities, were achieved (chi square test, p=0.03). The proportion of nitrate reductase assay ambiguous results was significantly higher in multidrug-resistant than in non-multidrug-resistant isolates (17.6% vs. 4.0%, chi square test, p<0.005). Conclusions.The nitrate reductase assay demonstrated provided reliable results for antibiotic resistance. However, using old cultures leds to a higher proportion of false sensitive results; furthermore, the nitrate reductase assay capability to detect multidrug-resistant tuberculosis decreased due to a higher proportion of non-interpretable results.es
dc.formatapplication/pdfES
dc.language.isoenes
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.sourceBiomédica, 2011, 31(2), 258–263.en_US
dc.subjectMycobacterium tuberculosises
dc.subjectResistencia a Medicamentoses
dc.subjectPruebas de Sensibilidad Microbianaes
dc.subjectMétodoses
dc.subjectNitrato-Reductasaes
dc.titleUtility of nitrate reductase assay for detection of multidrug-resistant Mycobacterium tuberculosis in a low resource settinges
dc.title.alternativeUtilidad del ensayo de nitrato reductasa en la detección de Mycobacterium tuberculosis multirresistente en caso de recursos limitadoses
dc.typeArtículoes
anlis.essnrd1es
item.openairetypeArtículo-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
crisitem.author.deptAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.deptInstituto Nacional de Enfermedades Respiratorias (INER)-
crisitem.author.deptDepartamento de Diagnóstico y Referencia-
crisitem.author.deptAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.deptInstituto Nacional de Enfermedades Respiratorias (INER)-
crisitem.author.deptDepartamento de Diagnóstico y Referencia-
crisitem.author.parentorgAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.parentorgInstituto Nacional de Enfermedades Respiratorias (INER)-
crisitem.author.parentorgAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.parentorgInstituto Nacional de Enfermedades Respiratorias (INER)-
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