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Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/355
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dc.contributor.authorLucero, Nidia E.-
dc.contributor.authorEscobar, Gabriela I.-
dc.contributor.authorAyala, Sandra M.-
dc.contributor.authorSilva Paulo, Patricia-
dc.contributor.authorNielsen, Klaus-
dc.date.accessioned2012-11-14T13:49:38Z-
dc.date.available2012-11-14T13:49:38Z-
dc.date.issued2003-
dc.identifier.issn0022-2615-
dc.identifier.urihttp://sgc.anlis.gob.ar/handle/123456789/355-
dc.identifier.urihttp://jmm.sgmjournals.org/content/52/10/883.full.pdf-
dc.descriptionFluorescence polarization immunoassay (FPA) uses molecular rotational properties to measure antibody binding toantigendirectly. Thepotential useof thismethodwas assessed incomparison to a competitive enzyme immunoassay (CELISA) and conventional serological tests for the diagnosis of brucellosis on a total of 587 human sera. Based on 340 sera from asymptomatic blood donors with no evidence of brucellosis, the specificity of the FPAwas97·9% using a cut-off value of 72mP. Sera fromBrucella-infected patients (11Brucellamelitensis,32Brucella abortus,32Brucella suis and oneBrucellasp.) yielded a sensitivity estimate of 96·1%. In tests on 84 sera from suspected brucellosis patients, the FPA detected 80 cases. Of 87 sera from patients with probable infection, 15 were detected by both CELISA and FPA, three by CELISA only and four by FPA only. The discrepancies inbothgroups involvedserawith low, declining titres. TheFPAuses a sampleof 40 l serum, takesabout 5 min tocompleteandhasbeendemonstrated tobeaccurate for thedetectionof antibodies toB. abortus, B. melitensisandB. suisand for identifying patients suffering relapses. Because of the ease of the procedure, it could be readily adopted for use in clinical laboratories and blood banks.en_US
dc.descriptionFil: Lucero, Nidia E. ANLIS Dr.C.G.Malbrán. Laboratorio de Brucelosis; Argentina.en_US
dc.descriptionFil: Escobar, Gabriela I. ANLIS Dr.C.G.Malbrán. Laboratorio de Brucelosis; Argentina.en_US
dc.descriptionFil: Ayala, Sandra M. ANLIS Dr.C.G.Malbrán. Laboratorio de Brucelosis; Argentina.en_US
dc.descriptionFil: Silva Paulo, Patricia, Comision Nacional de Energia Atomica. Centro Atomico de Ezeiza; Argentina.en_US
dc.descriptionFil: Nielsen, Klaus. Canadian Food Inspection Agency. Animal Diseases Research Institute; Canada.en_US
dc.formatapplication/pdfES
dc.language.isoengen_US
dc.rights2004-10-31en_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.sourceJournal of Medical Microbiology, 2003, 52(10), 883–887.en_US
dc.subjectBrucelosisen_US
dc.subjectDiagnósticoen_US
dc.subjectInmunoensayo de Polarización Fluorescenteen_US
dc.titleFluorescence polarization assay for diagnosis of human brucellosisen_US
dc.typeArtículoes
anlis.essnrd1es
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairetypeArtículo-
item.fulltextWith Fulltext-
item.languageiso639-1en-
crisitem.author.deptAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.deptInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.deptAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.deptInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.deptAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.deptInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.parentorgAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.parentorgAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.parentorgAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
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