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Title: | Multiplex PCR Assay for Identification of Human Diarrheagenic Escherichia coli | Authors: | Toma, Claudia Lu, Yan Higa, Naomi Nakasone, Noboru Chinen, Isabel Baschkier, Ariela Rivas, Marta Iwanaga, Masaaki |
Keywords: | Escherichia coli;Infecciones por Escherichia coli;Diarrea;Humanos;Reacción en Cadena de la Polimerasa Multiplex | Issue Date: | 2003 | Description: | A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli strain in a single reaction tube. Fil: Toma, Claudia. University of the Ryukyus. Department of Bacteriology; Japón. Fil: Lu, Yan. University of the Ryukyus. Department of Bacteriology; Japón. Fil: Higa, Naomi. University of the Ryukyus. Department of Bacteriology; Japón. Fil: Nakasone, Noboru.University of the Ryukyus. Department of Bacteriology; Japón. Fil: Chinen, Isabel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio de Fisiopatogenia; Argentina. Fil: Baschkier, Ariela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio de Fisiopatogenia; Argentina. Fil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio de Fisiopatogenia; Argentina. Fil: Iwanaga, Masaaki. University of the Ryukyus. Department of Bacteriology; Japón. |
URI: | http://sgc.anlis.gob.ar/handle/123456789/292 http://jcm.asm.org/content/41/6/2669.full.pdf+html |
ISSN: | 1098-660X | Rights: | info:eu-repo/semantics/openAccess |
Appears in Collections: | snrd Publicaciones INEI |
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File | Description | Size | Format | |
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JournalofClinicalMicrobiology,2003,41(6),2669–2671..pdf | 184.81 kB | Adobe PDF | View/Open |
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