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Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/269
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dc.contributor.authorPasteran, Fernando-
dc.contributor.authorMendez, Tania-
dc.contributor.authorRapoport, Melina J.-
dc.contributor.authorGuerriero, Leonor-
dc.contributor.authorCorso, Alejandra-
dc.date.accessioned2012-10-25T01:25:24Z-
dc.date.available2012-10-25T01:25:24Z-
dc.date.issued2010
dc.identifier.issn1098-660X
dc.identifier.urihttp://jcm.asm.org/content/48/4/1323.full.pdf+html-
dc.identifier.urihttp://sgc.anlis.gob.ar/handle/123456789/269-
dc.descriptionThe modified Hodge method (MHT) has been recommended by the CLSI for confirmation of suspected class A carbapenemase production in species of Enterobacteriaceae. This test and the Masuda method (MAS) have advantages over traditional phenotypic methods in that they directly analyze carbapenemase activity. In order to identify the potential interferences of these tests, we designed a panel composed of diverse bacterial genera with distinct carbapenem susceptibility patterns (42 carbapenemase producers and 48 nonproducers). About 25% of results among carbapenemase nonproducers, mainly strains harboring CTX-M and AmpC hyperproducers, were observed to be false positive. Subsequently, we developed an optimized approach for moreaccurate detection of suspicious isolates of carbapenemase by addition of boronic acid (BA) derivatives (reversible inhibitor of class A carbapenemases and AmpC cephalosporinases) and oxacillin (inhibitor of AmpCs enzymes). The use of the modified BA- and oxacillin-based MHT and MAS resulted in high sensitivity (>90%) and specificity (100%) for class A carbapenemase detection. By use of these methodologies, isolates producing KPCs and GES, Sme, IMI, and NMC-A carbapenemases were successfully distinguished from those producing other classes of ß-lactamases (extended-spectrum -lactamases [ESBLs], AmpC -lactamases, metallo- -lactamases [MBLs], etc.). These methods will provide the fast and useful information needed for targeting of antimicrobial therapy and appropriate infection control.ES
dc.descriptionFil: Pasteran, Fernando. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Antimicrobianos; Argentina.ES
dc.descriptionFil: Mendez, Tania. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Antimicrobianos; Argentina.ES
dc.descriptionFil: Rapoport, Melina J. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Antimicrobianos; Argentina.ES
dc.descriptionFil: Guerriero, Leonor. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Antimicrobianos; Argentina.ES
dc.descriptionFil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio de Antimicrobianos; Argentina.ES
dc.formatapplication/pdfES
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.sourceJournal of Clinical Microbiology, 2010, 48(4), 1323–1332.en_US
dc.subjectEnterobacteriaceaeen_US
dc.subjectReacciones Falso Positivasen_US
dc.subjectHumanosen_US
dc.subjectÁcidos Borónicosen_US
dc.subjectOxacilinaen_US
dc.titleControlling false-positive results obtained with the Hodge and Masuda assays for detection of class a carbapenemase in species of enterobacteriaceae by incorporating boronic Aciden_US
dc.typeArtículoes
anlis.essnrd1es
item.languageiso639-1en-
item.openairetypeArtículo-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextWith Fulltext-
item.grantfulltextopen-
crisitem.author.deptServicio Antimicrobianos-
crisitem.author.deptDepartamento de Bacteriología-
crisitem.author.deptInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.deptAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.deptServicio Antimicrobianos-
crisitem.author.deptDepartamento de Bacteriología-
crisitem.author.deptInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.deptAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.deptAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.deptInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.deptDepartamento de Bacteriología-
crisitem.author.deptServicio Antimicrobianos-
crisitem.author.parentorgDepartamento de Bacteriología-
crisitem.author.parentorgInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.parentorgAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.parentorgDepartamento de Bacteriología-
crisitem.author.parentorgInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.parentorgAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.parentorgAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.parentorgInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.parentorgDepartamento de Bacteriología-
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