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Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/248
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dc.contributor.authorCórdoba Lanús, Elizabeth-
dc.contributor.authorPiñero, José Enrique-
dc.contributor.authorGonzález, Ana Cristina-
dc.contributor.authorValladares, Basilio-
dc.contributor.authorLizarralde de Grosso, Mercedes-
dc.contributor.authorSalomón, Oscar Daniel-
dc.date.accessioned2012-10-21T01:54:43Z-
dc.date.available2012-10-21T01:54:43Z-
dc.date.issued2005-
dc.identifier.issn0074-0276-
dc.identifier.urihttp://sgc.anlis.gob.ar/handle/123456789/248-
dc.identifier.urihttp://www.scielo.br/pdf/mioc/v100n2/v100n2a13.pdf-
dc.descriptionAmerican cutaneous leishmaniasis (ACL) is an endemic disease in Northern Argentina. We applied the polymerase chain reaction (PCR) followed by a hybridization labelled probe to 21 paraffin embedded human skin biopsies, already analyzed histologically, from leishmaniasis endemic areas in the province of Tucumán, Argentina. We used primers previously designed to detect a Leishmania-specific 120-base-pair fragment of kinetoplast DNA minicircle, other two primer pairs that amplify kDNA minicircles belonging to the L. braziliensisand L. mexicana complexes respectively, and specific oligonucleotide primers to detect L. (V.) braziliensiswhich amplify the sequence of the ribosomal protein L-14 of this species. The PCR-hybridization showed a sensitivity of 90.5% when compared to the histopathology test which was 61.9%. Five of the total samples analyzed were positive for the L. braziliensis complex whilst none was positive for the L. mexicanacomplex. The specific primers for L. (V.) braziliensisdetected the parasite in four samples. These results are consistent with those reported for close endemic areas and demonstrate that the causative agent of human leishmaniasis in the analyzed cases was L. (V.) braziliensis. PCR should be used as a diagnostic tool for tegumentary leishmaniasis, especially in the mucosal form, and as a valuable technique for the identification of the Leishmania species that causes the disease in certain areas.en_US
dc.descriptionFil: Córdoba Lanús, Elizabeth. Universidad Nacional de Tucumán. Instituto Superior de Entomología “Dr. Abraham Willink”; Argentina.en_US
dc.descriptionFil: Piñero, José Enrique. Universidad de La Laguna. Departamento de Parasitología; Argentina.en_US
dc.descriptionFil: González, Ana Cristina. Universidad de La Laguna. Departamento de Parasitología; Argentina.en_US
dc.descriptionFil: Valladares, Basilio. Universidad de La Laguna. Departamento de Parasitología; Argentina.en_US
dc.descriptionFil: Lizarralde de Grosso, Mercedes. Universidad Nacional de Tucumán. Instituto Superior de Entomología “Dr. Abraham Willink”; Argentina.en_US
dc.descriptionFil: Salomón, Oscar Daniel. ANLIS Dr.C.G.Malbrán. Centro Nacional de Diagnóstico e Investigación en Endemo-Epidemias; Argentina.en_US
dc.formatapplication/pdfES
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.sourceMemórias do Instituto Oswaldo Cruz, 2005, 100(2), 187–192.en_US
dc.subjectLeishmania braziliensisen_US
dc.subjectLeishmaniasis Cutáneaen_US
dc.subjectReacción en Cadena de la Polimerasaen_US
dc.subjectArgentinaen_US
dc.subjectTucumánen_US
dc.titleDetection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reactionen_US
dc.typeArtículoes
dc.coverageARGen_US
dc.coverageTucumánen_US
anlis.essnrd1es
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairetypeArtículo-
item.fulltextWith Fulltext-
item.languageiso639-1en-
crisitem.author.deptAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.deptInstituto Nacional de Medicina Tropical (INMeT)-
crisitem.author.parentorgAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
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