Please use this identifier to cite or link to this item:
http://sgc.anlis.gob.ar/handle/123456789/1985
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Quelle, Liliana S | es |
dc.contributor.author | Corso, Alejandra | es |
dc.contributor.author | Galas, Marcelo F. | es |
dc.contributor.author | Sordelli, Daniel O. | es |
dc.date.accessioned | 2020-12-23T15:39:57Z | - |
dc.date.available | 2020-12-23T15:39:57Z | - |
dc.date.issued | 2003-11 | - |
dc.identifier.issn | 0732-8893 | - |
dc.identifier.uri | http://sgc.anlis.gob.ar/handle/123456789/1985 | - |
dc.description | Fil: Quelle, Liliana S. Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires; Argentina. | es |
dc.description | Fil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina. | es |
dc.description | Fil: Galas, Marcelo F. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina. | es |
dc.description | Fil: Sordelli, Daniel O. Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires; Argentina. | es |
dc.description.abstract | A method based on restriction profile analysis of the STAR repetitive element PCR (STAR-RP PCR) product obtained by digestion with AluI and Tru9I was developed for typing methicillin resistant Staphylococcus aureus (MRSA). We evaluated a well defined collection of MRSA from Argentina, previously characterized by PFGE (pulsed field gel electrophoresis) of chromosomal SmaI digests and hybridization with DNA probes for probes ClaI-mecA and ClaI-Tn554. We comparatively evaluated STAR-RP analysis with other PCR based methods such as Inter IS256-PCR, Rep-MP3 PCR and Coa-RP. The discriminatory power (D) of STAR-RP (0.86) was similar to that of PFGE (0.84) at the type level. Comparable results were obtained with Inter IS256 PCR (0.85) and Rep-MP3 PCR (0.80). A lower value (0.74) was obtained for Coa-RP. An excellent reproducibility (100%) of STAR-RP was observed. Good concordance between STAR-RP and other molecular typing methods was found for MRSA isolates (n = 39). STAR-RP typing showed 87% concordance with mecA::Tn554::PFGE, 87% with Inter IS256 PCR and 71% with Rep-MP3 typing. STAR-RP is suggested as an adequate molecular typing assay for MRSA epidemiologic assessment. | es |
dc.language.iso | en | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Diagnostic microbiology and infectious disease | es |
dc.rights | Closed Access | - |
dc.source | Diagnostic Microbiology and Infectious Disease 2003 ; 47(3):455-64. | - |
dc.subject | Staphylococcus aureus | es |
dc.title | STAR gene restriction profile analysis in epidemiological typing of methicillin-resistant Staphylococcus aureus: description of the new method and comparison with other polymerase chain reaction (PCR)-based methods | es |
dc.type | Artículo | es |
dc.identifier.doi | 10.1016/s0732-8893(03)00137-8 | - |
anlis.essnrd | 1 | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.fulltext | No Fulltext | - |
item.openairetype | Artículo | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | none | - |
crisitem.author.dept | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.dept | Instituto Nacional de Enfermedades Infecciosas (INEI) | - |
crisitem.author.dept | Departamento de Bacteriología | - |
crisitem.author.dept | Servicio Antimicrobianos | - |
crisitem.author.dept | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.dept | Instituto Nacional de Enfermedades Infecciosas (INEI) | - |
crisitem.author.parentorg | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.parentorg | Instituto Nacional de Enfermedades Infecciosas (INEI) | - |
crisitem.author.parentorg | Departamento de Bacteriología | - |
crisitem.author.parentorg | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
Appears in Collections: | Publicaciones INEI |
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