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http://sgc.anlis.gob.ar/handle/123456789/1734
DC Field | Value | Language |
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dc.contributor.author | Fellner, María Dolores | es |
dc.contributor.author | Durand, Karina | es |
dc.contributor.author | Correa, Mariel | es |
dc.contributor.author | Bes, David | es |
dc.contributor.author | Alonio, Lidia V. | es |
dc.contributor.author | Teyssie, Angelica R. | es |
dc.contributor.author | Picconi, María Alejandra | es |
dc.date.accessioned | 2020-11-24T23:08:16Z | - |
dc.date.available | 2020-11-24T23:08:16Z | - |
dc.date.issued | 2003-12 | - |
dc.identifier.issn | 1386-6532 | - |
dc.identifier.uri | http://sgc.anlis.gob.ar/handle/123456789/1734 | - |
dc.description | Fil: Fellner, María Dolores. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina. | es |
dc.description | Fil: Durand, Karina. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina. | es |
dc.description | Fil: Correa, Mariel. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina. | es |
dc.description | Fil: Bes, David. Hospital Nacional de Pediatría "Prof. Juan P. Garrahan"; Buenos Aires, Argentina. | es |
dc.description | Fil: Alonio, Lidia V. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina. | es |
dc.description | Fil: Teyssié, Angélica R. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina. | es |
dc.description | Fil: Picconi, María Alejandra. ANLIS Dr. C. G. Malbrán. Instituto Nacional de Enfermedades Infecciosas (INEI). Servicio Virus Oncogénicos; Argentina. | es |
dc.description.abstract | Background: High Epstein-Barr virus load has been related to an increased risk of Posttransplant Lymphoproliferative Disorders (PTLD) in transplant recipients. Objectives: Development of a method to quantitate EBV DNA levels in peripheral blood mononuclear cells (PBMC) and evaluate its usefulness in transplant patients. Study design: We designed a semiquantitative nested PCR based on a limiting dilution analysis to detect high viral loads in PBMC. This method was applied to 25 healthy carriers, and 85 solid organ transplant recipients as follows: (A) 53 asymptomatic patients; (B) 24 symptomatic patients; (C) eight patients with PTLD. Results: In healthy carriers the reciprocal of the limiting dilution (RLD) ranged between non-detected (ND) and 1, the median RLD was ND, which is equivalent to a viral load of <1 copy per 10(5) PBMC. In the transplant population the medians RLD (range) were: (A) asymptomatic group: ND (ND-64), median equivalent to a viral load of <1 copy per 10(5) PBMC; (B) symptomatic group: 4 (ND-256), median equivalent to a range of viral load of 4-64 copies per 10(5) PBMC. (C) PTLD group: 256 (16-16384), median equivalent to a range of viral load of 256-4096 copies per 10(5) PBMC. Statistically significant differences were found between all groups: A+B vs. C (P<0.0001); A vs. B (P<0.0001); A vs. C (P<0.0001), B vs. C (P<0.0001). We also observed a good correlation between viral loads and clinical findings in four follow-up patients. Considering the RLD=256 as a cutoff point to detect transplant patients with PTLD, resulted in sensitivity 75%, specificity 96.7%, positive predictive value 60%, negative predictive value 98.3%. Conclusion: This SQ-PCR method enables us to differentiate between transplant patients with and without PTLD; therefore, it could be applied as a marker for early detection of this pathology. | es |
dc.format | - | |
dc.language.iso | en | es |
dc.relation.ispartof | Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology | es |
dc.rights | Open Access | - |
dc.rights | Creative Commons Attribution 4.0 International License | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | Trastornos Linfoproliferativos | es |
dc.subject | Valor Predictivo de las Pruebas | es |
dc.subject | Trasplante de Hígado | es |
dc.subject | Carga Viral | es |
dc.title | A semiquantitative PCR method (SQ-PCR) to measure Epstein-Barr virus (EBV) load: its application in transplant patients | es |
dc.type | Artículo | es |
dc.identifier.doi | 10.1016/s1386-6532(03)00077-5 | - |
anlis.essnrd | 1 | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairetype | Artículo | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.dept | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.dept | Instituto Nacional de Enfermedades Infecciosas (INEI) | - |
crisitem.author.dept | Departamento de Virología | - |
crisitem.author.dept | Servicio Virus Oncogénicos | - |
crisitem.author.parentorg | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.parentorg | Instituto Nacional de Enfermedades Infecciosas (INEI) | - |
crisitem.author.parentorg | Departamento de Virología | - |
Appears in Collections: | Publicaciones INEI |
Files in This Item:
File | Description | Size | Format | |
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10.1016_S1386-6532(03)00077-5.pdf | Artículo en inglés | 232.76 kB | Adobe PDF | View/Open |
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