Please use this identifier to cite or link to this item:
http://sgc.anlis.gob.ar/handle/123456789/1378
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Vanasco, Bibiana N. | es |
dc.contributor.author | Jacob, Paulina | es |
dc.contributor.author | Landolt, Noelia | es |
dc.contributor.author | Chiani, Yosena | es |
dc.contributor.author | Schmeling, María Fernanda | es |
dc.contributor.author | Cudos, C | es |
dc.contributor.author | Tarabla, H | es |
dc.contributor.author | Lottersberger, Javier | es |
dc.date.accessioned | 2019-11-25T20:22:32Z | - |
dc.date.available | 2019-11-25T20:22:32Z | - |
dc.date.issued | 2016-04 | - |
dc.identifier.uri | http://sgc.anlis.gob.ar/handle/123456789/1378 | - |
dc.description.abstract | Enzyme-linked immunosorbent assay (ELISA) tests and polymerase chain reaction (PCR) may play a key role for early detection and treatment of human leptospirosis in developing countries. The aims of this study were to develop and validate an IgM ELISA under field conditions and to compare the diagnostic accuracy among IgG, IgM ELISAs, conventional PCR (cPCR), and real-time PCR (rtPCR) for early detection of human leptospirosis. Overall accuracy of IgM ELISA was sensitivity of 87.9%, specificity of 97.0%, and area under the curve of 0.940. When the 4 methods were compared, IgM ELISA showed the greatest diagnostic accuracy (J=0.6) followed by rtPCR (J=0.4), cPCR (J=0.2) and IgG ELISA (J=0.1). Our results support the use of IgM ELISA and rtPCR for early diagnosis of the disease. Moreover, due to their high specificity, they could be also useful to replace or supplement microscopic agglutination test as a confirmatory test, allowing more confirmations. | es |
dc.language.iso | en | es |
dc.relation.ispartof | Diagnostic microbiology and infectious disease | es |
dc.subject | IgG ELISA | es |
dc.subject | Métodos de diagnóstico | es |
dc.subject | Leptospirosis humana | es |
dc.subject | IgM ELISA | es |
dc.subject | Reacción en Cadena en Tiempo Real de la Polimerasa | es |
dc.title | Diagnostic accuracy of an IgM enzyme-linked immunosorbent assay and comparison with 2 polymerase chain reactions for early diagnosis of human leptospirosis | es |
dc.type | Artículo | es |
dc.identifier.doi | 10.1016/j.diagmicrobio.2016.01.002 | - |
item.openairetype | Artículo | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
crisitem.author.dept | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.dept | Instituto Nacional de Enfermedades Respiratorias (INER) | - |
crisitem.author.dept | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.dept | Instituto Nacional de Enfermedades Respiratorias (INER) | - |
crisitem.author.dept | Departamento de Diagnóstico y Referencia | - |
crisitem.author.dept | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.dept | Instituto Nacional de Enfermedades Respiratorias (INER) | - |
crisitem.author.dept | Departamento de Diagnóstico y Referencia | - |
crisitem.author.dept | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.dept | Instituto Nacional de Enfermedades Respiratorias (INER) | - |
crisitem.author.dept | Departamento de Diagnóstico y Referencia | - |
crisitem.author.dept | Maestría en Microbiología Molecular UNSM-ANLIS | - |
crisitem.author.dept | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.dept | Instituto Nacional de Enfermedades Respiratorias (INER) | - |
crisitem.author.dept | Departamento de Diagnóstico y Referencia | - |
crisitem.author.parentorg | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.parentorg | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.parentorg | Instituto Nacional de Enfermedades Respiratorias (INER) | - |
crisitem.author.parentorg | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.parentorg | Instituto Nacional de Enfermedades Respiratorias (INER) | - |
crisitem.author.parentorg | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.parentorg | Instituto Nacional de Enfermedades Respiratorias (INER) | - |
crisitem.author.parentorg | Centro Nacional Red de Laboratorios (CNRL) | - |
crisitem.author.parentorg | Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS) | - |
crisitem.author.parentorg | Instituto Nacional de Enfermedades Respiratorias (INER) | - |
Appears in Collections: | Publicaciones INER |
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