Please use this identifier to cite or link to this item: http://sgc.anlis.gob.ar/handle/123456789/1313
DC FieldValueLanguage
dc.contributor.authorTaverna, Constanza Giselleen_US
dc.contributor.authorMazza, Marianaen_US
dc.contributor.authorBueno, Nadia Soledaden_US
dc.contributor.authorAlvarez, Christianen_US
dc.contributor.authorAmigot, Susanaen_US
dc.contributor.authorAndreani, Marianaen_US
dc.contributor.authorAzula, Nataliaen_US
dc.contributor.authorBarrios, Rubénen_US
dc.contributor.authorFernández, Normaen_US
dc.contributor.authorFox, Barbaraen_US
dc.contributor.authorGuelfand, Lilianaen_US
dc.contributor.authorMaldonado, Ivanaen_US
dc.contributor.authorMurisengo, Omar Alejandroen_US
dc.contributor.authorRelloso, Silviaen_US
dc.contributor.authorVivot, Matias Ezequielen_US
dc.contributor.authorDavel, Graciela Odelsiaen_US
dc.date.accessioned2019-11-20T20:18:51Z-
dc.date.available2019-11-20T20:18:51Z-
dc.date.issued2019-02-01-
dc.identifier.urihttp://sgc.anlis.gob.ar/handle/123456789/1313-
dc.description.abstractMatrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has revolutionized the identification of microorganisms in clinical laboratories because it is rapid, relatively simple to use, accurate, and can be used for a wide number of microorganisms. Several studies have demonstrated the utility of this technique in the identification of yeasts; however, its performance is usually improved by the extension of the database. Here we developed an in-house database of 143 strains belonging to 42 yeast species in the MALDI Biotyper platform, and we validated the extended database with 388 regional strains and 15 reference strains belonging to 55 yeast species. We also performed an intra- and interlaboratory study to assess reproducibility and analyzed the use of the cutoff values of 1.700 and 2.000 to correctly identify at species level. The creation of an in-house database that extended the manufacturer's database was successful in view of no incorrect identification was introduced. The best performance was observed by using the extended database and a cutoff value of 1.700 with a sensitivity of .94 and specificity of .96. A reproducibility study showed utility to detect deviations and could be used for external quality control. The extended database was able to differentiate closely related species and it has potential in distinguishing the molecular genotypes of Cryptococcus neoformans and Cryptococcus gattii.en_US
dc.language.isoenen_US
dc.relation.ispartofMedical mycologyen_US
dc.titleDevelopment and validation of an extended database for yeast identification by MALDI-TOF MS in Argentinaen_US
dc.typeArtículoen_US
dc.identifier.doi10.1093/mmy/myy021-
item.openairetypeArtículo-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
crisitem.author.deptAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.deptInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.deptDepartamento de Micología-
crisitem.author.deptAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.deptInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.deptDepartamento de Micología-
crisitem.author.deptAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.deptInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.deptDepartamento de Micología-
crisitem.author.deptAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.deptInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.deptDepartamento de Micología-
crisitem.author.parentorgAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.parentorgInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.parentorgAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.parentorgInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.parentorgAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.parentorgInstituto Nacional de Enfermedades Infecciosas (INEI)-
crisitem.author.parentorgAdministración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán” (ANLIS)-
crisitem.author.parentorgInstituto Nacional de Enfermedades Infecciosas (INEI)-
Appears in Collections:Publicaciones INEI
Show simple item record

Page view(s)

90
checked on Mar 29, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.