http://sgc.anlis.gob.ar/handle/123456789/1158 INMeT 2026-04-23T04:47:51Z http://sgc.anlis.gob.ar/handle/123456789/2648 Title: Complete Genome Classification System of Rotavirus alphagastroenteritidis: An Updated Analysis Authors: Díaz Alarcón, Ricardo; Salvatierra, Karina; Gómez Quintero, Emiliano; Liotta, Domingo Javier; Parreño, Viviana; Miño, Samuel Abstract: Rotavirus alphagastroenteritidis is the major causative agent of acute gastroenteritis in both children under the age of 5 and young mammals and birds globally. RVAs are non-enveloped viruses with a genome comprising 11 double-stranded RNA segments. In 2008, the Rotavirus Classification Working Group pioneered a comprehensive and complete RVA genome classification system, establishing a specific threshold, which measures the genetic distances between homologous genes. The aim of this study was to perform an updated systematic analysis of the genetic variability across all RVA genes. Our investigation involved assessing the established cutoff values for each RVA genome segment and determining the need for any updates. To achieve this objective, multiple sequence alignments were constructed for all 11 genes and one for each genotype with discrepancies. Also, pairwise distances along with their cutoff values were evaluated. The analyses provided insights into the current relevance of cutoff values, which remain applicable for the majority of genotypes. In conclusion, this study fortifies the current classification system by highlighting its robustness and accurate genotyping of Rotavirus alphagastroenteritidis. Description: Fil: Díaz Alarcón RG. Laboratory of Applied Molecular Biology (LaBiMAp), Faculty of Exacts, Chemical and Natural Sciences, National University of Misiones (UNaM); Misiones; Argentina.; Fil: Salvatierra K. Laboratory "MADAR", National University of Misiones (UNaM); Misiones; Argentina.; Fil: Gómez Quintero. Laboratory of Applied Molecular Biology (LaBiMAp), Faculty of Exacts, Chemical and Natural Sciences, National University of Misiones (UNaM); Misiones; Argentina.; Fil: Liotta DJ. Laboratory of Applied Molecular Biology (LaBiMAp), Faculty of Exacts, Chemical and Natural Sciences, National University of Misiones (UNaM); Misiones; Argentina.; Fil: Parreño, V. National Institute of Agricultural Technology (INTA), IncuINTA, De Las Cabañas y De los Reseros s/n; Buenos Aires; Argentina.; Fil: Miño SO. Laboratory of Applied Molecular Biology (LaBiMAp), Faculty of Exacts, Chemical and Natural Sciences, National University of Misiones (UNaM); Misiones; Argentina. 2025-01-31T00:00:00Z http://sgc.anlis.gob.ar/handle/123456789/2631 Title: Laboratory Performance of Cactoblastis cactorum (Lepidoptera: Pyralidae) on South and North American Opuntia Species Occurring in Argentina Authors: Varone, Laura; Manteca Acosta, Mariana; Logarzo, Guillermo; Briano, Juan; Hight, Stephen; Carpenter, James Abstract: The cactus moth, Cactoblastis cactorum (Berg), is native to South America. Since its unintentional arrival to the United States in 1989 and to Mexican islands in 2006, it has become a serious threat to the diversity of both wild and cultivated species of Opuntia Mill. in North America. The native ecological host range of C. cactorum has not been directly ascertained and host acceptance is unclear. Taxonomic nomenclature of Opuntia spp. has been confusing, contradictory, and rapidly changing, leading to inaccurate conclusions about host plant use by C. cactorum in its native South American range. This study was conducted to better understand the biology and ecology of C. cactorum in Argentina by evaluating, under laboratory conditions, the insects’ performance (survivorship, development time, potential fecundity) on 8 Opuntia spp. occurring in Argentina. Feeding trials were conducted on 5 Opuntia spp. native to Argentina and 3 Opuntia spp. native to Mexico. Cactoblastis cactorum larvae failed to feed on 2 native Opuntia spp., and had their greatest performance on the North American O. ficus-indica (L). Mill. and O. robusta H. L. Wendl. ex Pfeiff., and the South American O. arechavaletae Speg. Because the insects for the experiments were originally collected on O. ficus-indica, a reciprocal cross feeding experiment with insects collected on O. megapotamica Arechav. was also conducted to test for a potential host plantmediated local adaptation effect. Some evidence for host plant adaptation was detected in populations collected on the South American host, O. megapotamica. Local adaptation, as documented here, could have consequences for the invasion process of C. cactorum in North America. Description: Fil: Varone, L. Fundación para el Estudio de Especies Invasivas (FUEDEI); Hurlingham, Buenos aires, Argentina.; Fil: Manteca Acosta, M. Instituto Nacional de Medicina Tropical (INMeT), Ministerio de Salud de la Nación; Puerto Iguazú, Misiones, Argentina.; Fil: Logarzo, G. Fundación para el Estudio de Especies Invasivas (FUEDEI); Hurlingham, Buenos aires, Argentina.; Fil: Briano, J. Fundación para el Estudio de Especies Invasivas (FUEDEI); Hurlingham, Buenos aires, Argentina.; Fil: Hight, S. USDA-ARS-CMAVE, FAMU; Florida, Estados Unidos; Fil: Carpenter, J. USDA-ARS-CPMRU; Tifton, Estados Unidos 2012-12-01T00:00:00Z http://sgc.anlis.gob.ar/handle/123456789/2629 Title: First description of Migonemyia migonei (França) and Nyssomyia whitmani (Antunes & Coutinho) (Psychodidae: Phlebotominae) natural infected by Leishmania infantum in Argentina Authors: Moya, Sofía Lorían; Giuliani, Magalí; Manteca Acosta, Mariana; Salomón, Oscar Daniel; Liotta, Domingo Javier Abstract: Leishmania infantum is the etiological agent of the Visceral Leishmaniasis (VL) disease in America, with Lutzomyia longipalpis phlebotomine sandflies as its proven vectors in Argentina, and infected dogs as its main urban reservoir. In Puerto Iguazú City (Misiones province, Argentina), human and canine cases of VL were recorded. Additionally, in the rural area known as "2000 Hectáreas", less than 10km away from the city, several human cases of Tegumentary Leishmaniasis (TL) were registered determining an endemic area with Leishmania braziliensis as the etiological agent. Because of this, several phlebotomine captures were done in this site showing that Nyssomyia whitmani is the most abundant sandfly followed by Migonemyia migonei. In this study, three of the sandflies captured were found infected whit L. infantum parasites, detected by PCR and sequencing. Two of them were N. whitmani and the other one was a M. migonei specimen, being this the first report of L. infantum natural infection for Argentina in these sandfly species. N. whitmani is the main vector of L. braziliensis in this area, and M. migonei has been suggested as a putative vector in other locations where human and canine cases of VL where reported with L. longipalpis apparently absent. In this context, we consider necessary further studies that could define the role of M. migonei and N. whitmani as specific or permissive vectors of L. infantum, their vectorial competence and capacity, and their actual role in the transmission of both Tegumentary and Visceral Leishmaniasis in the study area. Description: Fil: Moya S. Instituto Nacional de Medicina Tropical (INMeT-MSAL), Jujuy y Neuquén s/n, Pto. Iguazú, Misiones, Argentina; Laboratorio de Biología Molecular Aplicada (LaBiMAp-FCEQyN-UNaM); Posadas, Misiones, Argentina; Fil: Giuliani, M. Instituto Nacional de Medicina Tropical (INMeT-MSAL), Jujuy y Neuquén s/n, Pto. Iguazú, Misiones, Argentina; Laboratorio de Biología Molecular Aplicada (LaBiMAp-FCEQyN-UNaM); Posadas, Misiones, Argentina; Fil: Manteca Acosta, M. Instituto Nacional de Medicina Tropical (INMeT-MSAL); Iguazú, Misiones, Argentina; Fil: Salomón, O. Instituto Nacional de Medicina Tropical (INMeT-MSAL); Iguazú, Misiones, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina; Fil: Liotta D. Instituto Nacional de Medicina Tropical (INMeT-MSAL); Iguazú, Misiones, Argentina; Laboratorio de Biología Molecular Aplicada (LaBiMAp-FCEQyN-UNaM); Posadas, Misiones, Argentina 2015-12-01T00:00:00Z http://sgc.anlis.gob.ar/handle/123456789/2580 Title: Pintomyia evansi (Diptera: Psychodidae) larvae susceptibility to hydrated lime under laboratory conditions Authors: Ortega, Edgar; Estrada, Luis Gregorio; Bejarano, Eduar Elías; Cadena, Horacio Abstract: This study evaluated the effect of Calcium hydroxide (Ca(OH)2 Mg(OH)2) on third stages Pi. evansi larvae mortality under experimental laboratory conditions. Three treatments containing a mixture of phlebotomine natural breeding soil (substrate) and Calcium hydroxide at different concentrations were used: Treatment 1 (T1), 1 kg of substrate mixed with 56.2 g of lime; Treatment 2 (T2), 1 kg of substrate mixed with 62.5 g of lime; and Treatment 3 (T3), 1 kg of substrate mixed with 70 g of lime. in addition, a sample of substrate without lime was used as a control for each treatment. The mortality in T1 was 1% at 24 h and 12% at 48 h, reaching a maximum of 56% at 72 h of exposure. For T2, mortality was progressive, starting with 12% at 12 h, 36% at 24 h, 52% at 48 h, and 100% at 72 h; while T3 showed mortality percentages of 94% and 100% between 12 and 24 h of exposure. Therefore, T3 was the most effective to according to the Kaplan-Meier survival analysis. This study showed that treatments over 62 g of Calcium hydroxide per 1 kg of substrate offer a starting point for immature stage control under laboratory conditions. With these results, we propose to evaluate the cost-effectiveness and feasibility of the application, of the latter concentration, under field conditions in urban environments for its application in vector control programs. Description: Fil: Ortega, Edgar. Investigaciones Biomédicas, Universidad de Sucre; Colombia; Fil: Estrada, Luis Gregorio. Instituto Nacional de Medicina Tropical, ANLIS-Malbrán, Ministerio de Salud de la Nación. Almafuerte y Ámbar s/n; Argentina; Fil: Bejarano, Eduar Elías. Investigaciones Biomédicas, Universidad de Sucre; Colombia; Fil: Cadena, Horacio. Programa de Estudio y Control de Enfermedades Tropicales - PECET, Universidad de Antioquia; Colombia 2022-08-01T00:00:00Z