The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material. http://sgc.anlis.gob.ar:80 2026-06-06T08:50:28Z 2026-06-06T08:50:28Z Bidondo, María Paz Brun, Paloma Groisman, Boris Trotta, Marianela Barbero, Pablo http://sgc.anlis.gob.ar/handle/123456789/2756 2026-05-27T20:17:25Z 2026-05-06T00:00:00Z

Title: Teratogenic Effect of OROV Infection: An Analysis of Its Causality Authors: Bidondo, María Paz; Brun, Paloma; Groisman, Boris; Trotta, Marianela; Barbero, Pablo Abstract: Oropouche virus (OROV) is an arbovirus that has caused multiple epidemic outbreaks in several Latin American countries. In 2024, cases of possible vertical transmission with congenital microcephaly were reported, raising concerns about potential teratogenic effects. However, the causal relationship between OROV infection during pregnancy and congenital anomalies remains poorly defined. This study aimed to analyze the evidence on the potential teratogenic effect of OROV in humans through a systematic literature review, applying Shepard's criteria for causality. The available evidence supports a temporal relationship between exposure during critical periods of prenatal development and observed effects in offspring. Reported cases share certain common characteristics that could suggest a specific syndrome. Experimental studies in animal models demonstrate vertical transmission of OROV and harmful effects in offspring. Considering the documented vertical transmission, viral presence in the central nervous system, and known pathogenic mechanisms, biological plausibility exists for a teratogenic effect. However, no epidemiological study has yet evaluated the association between OROV infection and teratogenicity. Furthermore, given that infection occurs during epidemic outbreaks rather than through sporadic environmental exposure, the criterion of infrequent exposure associated with an infrequent defect is not met. Therefore, Shepard's teratogenicity criteria are not yet fully fulfilled. In conclusion, while suggestive evidence exists, definitive proof of OROV teratogenicity remains lacking. Health authorities should prioritize research initiatives, strengthen epidemiological surveillance groups, ensure availability of serological testing for OROV infection, and enhance congenital defect surveillance systems to better characterize this potential association.

2026-05-06T00:00:00Z Traglia, German M Moheb, Samyar Akhar, Usman Quiroga, Cecilia Tuttobene, Marisel R Mase, Hamza Pasteran, Fernando Rao, Gauri G. Tolmasky, Marcelo E. Bonomo, Robert A Ramirez, Maria Soledad http://sgc.anlis.gob.ar/handle/123456789/2755 2026-05-27T20:17:09Z 2026-05-12T00:00:00Z

Title: Impact of Cefiderocol on Klebsiella pneumoniae: A Population-based Longitudinal Analysis of Phenotypic and Genotypic Responses at Subinhibitory and Inhibitory Concentrations Authors: Traglia, German M; Moheb, Samyar; Akhar, Usman; Quiroga, Cecilia; Tuttobene, Marisel R; Mase, Hamza; Pasteran, Fernando; Rao, Gauri G.; Tolmasky, Marcelo E.; Bonomo, Robert A; Ramirez, Maria Soledad Abstract: Background: Cefiderocol (FDC) is a siderophore cephalosporin active against carbapenem-resistant Klebsiella pneumoniae (CRKP), but resistance has emerged, often involving iron-uptake pathways and β-lactamases. We investigated how an NDM-producing clinical isolate adapts to FDC under stepwise selective pressure. Methods: A ST147 K. pneumoniae isolate (Kp-1) carrying blaNDM-5 and blaOXA-181, susceptible to FDC but resistant to other agents, was propagated in iron-depleted Mueller-Hinton broth with progressive FDC exposure. Population-based whole-genome sequencing (WGS) tracked mutation dynamics, and RT-qPCR profiled 17 genes related to FDC resistance, iron acquisition, and virulence, comparing the parental population (G0) to the first exposed generation (G1 + FDC). Results: The mean number of mutations detected in generation G1 under FDC exposure was 24.3 ± 12.2, and in generation G2, it increased to 31.7 ± 6.4. Two nonsynonymous substitutions in baeS (V295G and T299P) were recurrently selected, with V295G reaching ≥ 70% frequency in independent lineages, indicating convergent evolution. RT-qPCR revealed downregulation of iron-uptake and porin genes (iroN, cirA, fepA, kfu, ompK35), and upregulation of entB, ompK36, blaNDM-5, and capsule-related genes (wzm, wbbM); baeS/baeR transcript levels were unchanged. Early FDC exposure enhanced biofilm formation without significantly affecting capsule production. Conclusions: Short-term FDC exposure reproducibly selects baeS variants and triggers a transcriptional program reducing siderophore-receptor entry and remodeling the outer membrane. These adaptations-together with increased blaNDM-5 expression and biofilm formation-contribute to reduced FDC susceptibility. The recurrent baeS mutations (V295G/T299P) and decreased cirA/fepA expression may serve as early surveillance markers of FDC adaptation in K. pneumoniae.

2026-05-12T00:00:00Z Lavayén, Silvina Silva, Andrea Cejas, Luciana Córdoba, Marcelo Daniel Breit, Dolores http://sgc.anlis.gob.ar/handle/123456789/2754 2026-05-26T18:04:08Z 2026-01-01T00:00:00Z

Title: Revisión de los casos notificados en SIVILE 2023 a través del campo "Observaciones" Authors: Lavayén, Silvina; Silva, Andrea; Cejas, Luciana; Córdoba, Marcelo Daniel; Breit, Dolores Abstract: El Informe tuvo como objetivo realizar una revisión exhaustiva de los casos registrados en el Sistema de Vigilancia de Lesiones (SIVILE) durante el año 2023, con el fin de mejorar la calidad del registro mediante el análisis del campo cualitativo de "Observaciones". Se buscó enriquecer la base de datos y proporcionar recomendaciones para optimizar el manual de registro. Description: Fil: Lavayen, Silvina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Epidemiología; Argentina.; Fil: Silva, Andrea. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Epidemiología; Argentina.; Fil: Cejas, Luciana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Epidemiología; Argentina.; Fil: Córdoba, Marcelo Daniel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Epidemiología; Argentina.; Fil: Breit, Dolores. Programa Nacional de Prevención y Control de Lesiones por Causas Externas. Ministerio de Salud de la Nación; Argentina.

2026-01-01T00:00:00Z Lerman, Andrea Navarro Albarracín, Florencia Fígari, Alejandra Macias Lainez, Valeria Uez, Osvaldo http://sgc.anlis.gob.ar/handle/123456789/2753 2026-05-26T17:49:37Z 2023-01-01T00:00:00Z

Title: Rinovirus y metapneumovirus en pacientes con infección respiratoria aguda grave Authors: Lerman, Andrea; Navarro Albarracín, Florencia; Fígari, Alejandra; Macias Lainez, Valeria; Uez, Osvaldo Abstract: El presente trabajo describe la frecuencia de rinovirus y metapneumovirus en pacientes pediátricos de una unidad centinela de Mar del Plata con infección respiratoria aguda grave y resultado negativo para virus clásicos por inmunofluorescencia y biología molecular. Se realizó un estudio descriptivo de corte transversal. Se evaluó la presencia de rinovirus y metapneumovirus por biología molecular en 163 casos negativos para panel respiratorio por técnicas de vigilancia referencial, durante todo el año 2015. Se detectó rinovirus en el 51,5 % de los casos, metapneumovirus en el 9,8 % y coinfección rinovirus-metapneumovirus en el 6,1 %. Fueron negativos para ambos virus el 32,5 %. Description: Fil: Lerman, Andrea. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Epidemiología Dr. Juan H. Jara. Laboratorio de Diagnóstico y Referencia; Argentina.; Fil: Navarro Albarracín, Florencia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Epidemiología Dr. Juan H. Jara. Laboratorio de Diagnóstico y Referencia; Argentina.; Fil: Fígari, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Epidemiología Dr. Juan H. Jara. Laboratorio de Diagnóstico y Referencia; Argentina.; Fil: Macias Lainez, Valeria. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Epidemiología Dr. Juan H. Jara. Laboratorio de Diagnóstico y Referencia; Argentina.; Fil: Uez, Osvaldo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Epidemiología Dr. Juan H. Jara. Laboratorio de Diagnóstico y Referencia; Argentina.

2023-01-01T00:00:00Z